Differential expression and role of two dithiol glutaredoxins Grx1 and Grx2 in Schizosaccharomyces pombe.
Identifieur interne : 000E85 ( Main/Exploration ); précédent : 000E84; suivant : 000E86Differential expression and role of two dithiol glutaredoxins Grx1 and Grx2 in Schizosaccharomyces pombe.
Auteurs : Woo-Hyun Chung [Corée du Sud] ; Kyoung-Dong Kim ; Yong-Joon Cho ; Jung-Hye RoeSource :
- Biochemical and biophysical research communications [ 0006-291X ] ; 2004.
Descripteurs français
- KwdFr :
- Cytosol (enzymologie), Expression des gènes (MeSH), Glutarédoxines (MeSH), Gènes fongiques (MeSH), Gènes fongiques du type conjugant (MeSH), Microscopie de fluorescence (MeSH), Mitochondries (enzymologie), Mutation (MeSH), Oxidoreductases (génétique), Oxidoreductases (métabolisme), Protéines associées à la pancréatite (MeSH), Schizosaccharomyces (enzymologie), Schizosaccharomyces (génétique), Stress oxydatif (MeSH).
- MESH :
- enzymologie : Cytosol, Mitochondries, Schizosaccharomyces.
- génétique : Oxidoreductases, Schizosaccharomyces.
- métabolisme : Oxidoreductases.
- Expression des gènes, Glutarédoxines, Gènes fongiques, Gènes fongiques du type conjugant, Microscopie de fluorescence, Mutation, Protéines associées à la pancréatite, Stress oxydatif.
English descriptors
- KwdEn :
- Cytosol (enzymology), Gene Expression (MeSH), Genes, Fungal (MeSH), Genes, Mating Type, Fungal (MeSH), Glutaredoxins (MeSH), Microscopy, Fluorescence (MeSH), Mitochondria (enzymology), Mutation (MeSH), Oxidative Stress (MeSH), Oxidoreductases (genetics), Oxidoreductases (metabolism), Pancreatitis-Associated Proteins (MeSH), Schizosaccharomyces (enzymology), Schizosaccharomyces (genetics).
- MESH :
- chemical , genetics : Oxidoreductases.
- chemical , metabolism : Oxidoreductases.
- chemical : Glutaredoxins, Pancreatitis-Associated Proteins.
- enzymology : Cytosol, Mitochondria, Schizosaccharomyces.
- genetics : Schizosaccharomyces.
- Gene Expression, Genes, Fungal, Genes, Mating Type, Fungal, Microscopy, Fluorescence, Mutation, Oxidative Stress.
Abstract
Glutaredoxins are glutathione-specific thiol oxidoreductases. The regulation and the role of grx1(+) and grx2(+) genes encoding dithiol glutaredoxins were analyzed in Schizosaccharomyces pombe. When tested in the same genetic background including mating type, the grx1 null mutant became sensitive to hydrogen peroxide, whereas grx2 mutant became highly sensitive to paraquat, a superoxide generator. The grx1grx2 double mutant showed additive phenotype of each single mutant. The grx1(+) gene expression was induced by various stresses such as oxidants, salts, and heat, and increased in the stationary phase, whereas grx2(+) stayed constitutive. The induction was mediated via Spc1 MAP kinase path involving both Atf1 and Pap1 transcription factors. Sub-cellular fractionation as well as fluorescence microscopy revealed that Grx1 resides mainly in the cytosol, whereas Grx2 is in mitochondria. These results suggest distinct roles for Grx1 and Grx2 in S. pombe in mediating glutathione-dependent redox homeostasis.
DOI: 10.1016/j.bbrc.2004.07.042
PubMed: 15358115
Affiliations:
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(MeSH)</term><term>Microscopy, Fluorescence (MeSH)</term><term>Mitochondria (enzymology)</term><term>Mutation (MeSH)</term><term>Oxidative Stress (MeSH)</term><term>Oxidoreductases (genetics)</term><term>Oxidoreductases (metabolism)</term><term>Pancreatitis-Associated Proteins (MeSH)</term><term>Schizosaccharomyces (enzymology)</term><term>Schizosaccharomyces (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cytosol (enzymologie)</term><term>Expression des gènes (MeSH)</term><term>Glutarédoxines (MeSH)</term><term>Gènes fongiques (MeSH)</term><term>Gènes fongiques du type conjugant (MeSH)</term><term>Microscopie de fluorescence (MeSH)</term><term>Mitochondries (enzymologie)</term><term>Mutation (MeSH)</term><term>Oxidoreductases (génétique)</term><term>Oxidoreductases (métabolisme)</term><term>Protéines associées à la pancréatite (MeSH)</term><term>Schizosaccharomyces (enzymologie)</term><term>Schizosaccharomyces (génétique)</term><term>Stress oxydatif 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The regulation and the role of grx1(+) and grx2(+) genes encoding dithiol glutaredoxins were analyzed in Schizosaccharomyces pombe. When tested in the same genetic background including mating type, the grx1 null mutant became sensitive to hydrogen peroxide, whereas grx2 mutant became highly sensitive to paraquat, a superoxide generator. The grx1grx2 double mutant showed additive phenotype of each single mutant. The grx1(+) gene expression was induced by various stresses such as oxidants, salts, and heat, and increased in the stationary phase, whereas grx2(+) stayed constitutive. The induction was mediated via Spc1 MAP kinase path involving both Atf1 and Pap1 transcription factors. Sub-cellular fractionation as well as fluorescence microscopy revealed that Grx1 resides mainly in the cytosol, whereas Grx2 is in mitochondria. These results suggest distinct roles for Grx1 and Grx2 in S. pombe in mediating glutathione-dependent redox homeostasis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15358115</PMID><DateCompleted><Year>2004</Year><Month>10</Month><Day>14</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0006-291X</ISSN><JournalIssue CitedMedium="Print"><Volume>321</Volume><Issue>4</Issue><PubDate><Year>2004</Year><Month>Sep</Month><Day>03</Day></PubDate></JournalIssue><Title>Biochemical and biophysical research communications</Title><ISOAbbreviation>Biochem Biophys Res Commun</ISOAbbreviation></Journal><ArticleTitle>Differential expression and role of two dithiol glutaredoxins Grx1 and Grx2 in Schizosaccharomyces pombe.</ArticleTitle><Pagination><MedlinePgn>922-9</MedlinePgn></Pagination><Abstract><AbstractText>Glutaredoxins are glutathione-specific thiol oxidoreductases. The regulation and the role of grx1(+) and grx2(+) genes encoding dithiol glutaredoxins were analyzed in Schizosaccharomyces pombe. When tested in the same genetic background including mating type, the grx1 null mutant became sensitive to hydrogen peroxide, whereas grx2 mutant became highly sensitive to paraquat, a superoxide generator. The grx1grx2 double mutant showed additive phenotype of each single mutant. The grx1(+) gene expression was induced by various stresses such as oxidants, salts, and heat, and increased in the stationary phase, whereas grx2(+) stayed constitutive. The induction was mediated via Spc1 MAP kinase path involving both Atf1 and Pap1 transcription factors. Sub-cellular fractionation as well as fluorescence microscopy revealed that Grx1 resides mainly in the cytosol, whereas Grx2 is in mitochondria. 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PubStatus="medline"><Year>2004</Year><Month>10</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>9</Month><Day>11</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15358115</ArticleId><ArticleId IdType="doi">10.1016/j.bbrc.2004.07.042</ArticleId><ArticleId IdType="pii">S0006-291X(04)01483-4</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement><orgName><li>Université nationale de Séoul</li></orgName></list><tree><noCountry><name sortKey="Cho, Yong Joon" sort="Cho, Yong Joon" uniqKey="Cho Y" first="Yong-Joon" last="Cho">Yong-Joon Cho</name><name sortKey="Kim, Kyoung Dong" sort="Kim, Kyoung Dong" uniqKey="Kim K" first="Kyoung-Dong" last="Kim">Kyoung-Dong Kim</name><name sortKey="Roe, Jung Hye" sort="Roe, Jung Hye" uniqKey="Roe J" first="Jung-Hye" last="Roe">Jung-Hye Roe</name></noCountry><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Chung, Woo Hyun" sort="Chung, Woo Hyun" uniqKey="Chung W" first="Woo-Hyun" last="Chung">Woo-Hyun Chung</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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